Immunophenotyping laboratory was set up in August 2013 under the patronage of RA Ministry of Health, this laboratory is considered new department not only in Hematology center but also in this region.
This laboratory provides advanced flow cytometric service at cellular and subcellular levels. Immunophenotyping has revolutionized the diagnosis of acute leukemia by generating increasingly detailed and prognostically significant phenotypes. Response to therapy, residual disease post-therapy and early relapse is all readily assayed by flow cytometry.
The laboratory uses BD FACSCalibur 4 colors analyzers to process the samples. Our model has a 488 nm blue laser allowing 3 colors detection (530/30, 575/26, and 650LP filters), and a 635 nm red laser that enables 1 color detection (670LP filter). The patient`s cells are stained with monoclonal antibodies (Becton, Dickinson and BD Biosciences) specific to both surface and intracellular antigens, in order that a diagnosis is obtained in accordance with World Health Organization (WHO) guidelines.
Specimens used for the test:
- Peripheral blood
- Bone Marrow
- Cerebrospinal fluid (CSF)
- Body Fluids
Tests:
1. Immunophenotyping for all types of leukemia and lymphoma:
- Chronic Myelogenous Leukemia (CML)
- Acute Myeloid Leukemia (AML)
- Acute Myeloblastic Leukemia
- Acute Myelomonocytic Leukemia
- Acute Lymphoblastic/Lymphocytic Leukemia
- Mixed Lineage leukemia (MLL)
- Acute Promyelocytic Leukemia (APL)
- Acute Erythroid Leukemia
- Precursor B-Lymphoblastic Leukemia
- Precursor T-Lymphoblastic Leukemia
- Chronic Lymphocytic Leukemia of B-Cell (B-CLL)
- Chronic Lymphocytic Leukemia of T-Cell (T-CLL)
- Small Lymphocytic Lymphoma (SLL)
- Prolymphocytic leukemia (PLL)
- Hairy cell leukemia (HCL)
- Multiple Myeloma (MM)
- Myelodysplastic syndromes
- Mature T and NK cell neoplasms
- Hodjkin Lymphoma
2. Detection of minimal residual disease (MRD) for Acute Leukemia.
3. Enumeration of lymphocyte subsets, stem cells.
4. HLADr Cross-matching by flow cytometry for kidney transplantation.
5. Post transplantation monitoring.
- T-cell markers: CD3, CD4, CD8
- B-cell markers: CD19
- NK-cell markers: CD56
6. Early diagnosis of breast cancer by Flowcytometric analysis
7. Platelets function testing.
Cytogenetic laboratory
- Detection of the Philadelphian chromosome BCR/ABL t (9; 22)
- Bone-marrow karyotyping
- Blood karyotyping
- AML diagnosis by FISH method and Karyotyping
- ALL diagnosis by FISH method and Karyotyping
- CLL diagnosis by FISH method
- Myeloma diagnosis by FISH method
- Lymphoma diagnosis by FISH method
- Detection of the PML/RARA translocation during APL
- AL diagnosis by FISH method and Karyotyping
- Detection of the FIP1L/CHIC2/PDGFRA deletion
- Analysis of amniotic fluid and chorionic villi by FISH method
- Detection of the Her-2 mutation in patients with breast cancer
- Detection of the Hemophilia Factor VIII gene (Xq28) mutation by FISH method
- Blood karyotyping in couples suffering from infertility
Laboratory of Molecular Genetics
- Detection of Jak-2 gene by allele-specific PCR method
- Qualitative detection of BCR/ABL translocation by PCR method
- Quantitative detection of BCR/ABL translocation by PCR method
Method of electrophoresis
- Hemoglobin electrophoresis for detection A, A2, F and abnormal types of hemoglobin by Helena V8 automatic analyzer
- Serum protein immune capillary electrophoresis by Helena V8 automatic analyzer
- Serum protein capillary electrophoresis by Helena V8 automatic analyzer
- Urine protein capillary electrophoresis by Helena V8 automatic analyzer
- Biological fluids capillary electrophoresis by Helena V8 automatic analyzer
- Bens Johns pathological protein detection in urine and serum using semi HYDRASYS 2 scan Sebia analyzer